Controlling Liquid–Liquid Phase Separation of Cold-Adapted Crystallin Proteins from the Antarctic Toothfish
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چکیده
منابع مشابه
γS-Crystallin Proteins from the Antarctic Nototheniid Toothfish: A Model System for Investigating Differential Resistance to Chemical and Thermal Denaturation
The γS1- and γS2-crystallins, structural eye lens proteins from the Antarctic toothfish (Dissostichus mawsoni), are homologues of the human lens protein γS-crystallin. Although γS1 has the higher thermal stability of the two, it is more susceptible to chemical denaturation by urea. The lower thermodynamic stability of both toothfish crystallins relative to human γS-crystallin is consistent with...
متن کاملCold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis.
The beta-galactosidase from the Antarctic gram-negative bacterium Pseudoalteromonas haloplanktis TAE 79 was purified to homogeneity. The nucleotide sequence and the NH(2)-terminal amino acid sequence of the purified enzyme indicate that the beta-galactosidase subunit is composed of 1,038 amino acids with a calculated M(r) of 118,068. This beta-galactosidase shares structural properties with Esc...
متن کاملNovel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome
An Antarctic soil metagenomic library was screened for lipolytic enzymes and allowed for the isolation of a new cytosolic esterase from the a/b hydrolase family 6, named MHlip. This enzyme is related to hypothetical genes coding esterases, aryl-esterases and peroxydases, among others. MHlip was produced, purified and its activity was determined. The substrate profile of MHlip reveals a high spe...
متن کاملCold-stable eye lens crystallins of the Antarctic nototheniid toothfish Dissostichus mawsoni Norman.
The eye lenses of the Antarctic nototheniid fishes that inhabit the perennially freezing Antarctic seawater are transparent at -2 degrees C, whereas the cold-sensitive mammalian and tropical fish lenses display cold-induced cataract at 20 degrees C and 7 degrees C, respectively. No cold-cataract occurs in the giant Antarctic toothfish Dissostichus mawsoni lens when cooled to temperatures as low...
متن کاملUnnatural Selection of Antarctic Toothfish in the Ross Sea, Antarctica
Historically, fishermen targeted fish in shallow, nearshore waters relatively close to port (Pauly et al. 2005). As these species became depleted, to meet growing demands, fishermen were forced to move offshore and into deeper waters (Hutchings and Reynolds 2004; Koslow et al. 2000; Morato et al. 2006; Hilborn et al. 2003; Pauly et al. 2002, 2005). Steady advances in fishing technology facilita...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2018
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2018.10.023